10 VIRULENT AND ATTENUATED POLIOVIRUSES Attenuated
نویسنده
چکیده
This paper is concerned with the correlation of an in ~tro marker of poliovirus particles, the capacity for multiplication on a monkey stable (MS) cell line, with thc degree of ncurovirulence for monkeys. Vogt a M. (I) discovered that the plaque formation of most attenuated strains of poliovirus is delaycd or inhibited, when the amount of bicarbonate in the agar overlay is reduced. However, a number of attenuated strains of the delayed, or d, type have becn shown to be capable of revcrsion to the wild, #type, without markedly changing their low degree of neurovirulence for monkeys (2, 3). The present authors have recently discovered another marker which may be used to differentiate poliovirus particles. Virulent strains grow well on primary monkey kidney cultures and on the MS ccll line? Attenuated strains grow poorly and form very tiny plaques, or none at all, on MS cells. They arc classified as MS mutants , and are said to possess the MS character, in contrast to the MS + marker of wild, virulent polioviruses.
منابع مشابه
An Aluminum Marker for the Differentiation and Separation of Virulent and Attenuated Polioviruses
A new character, the A marker, for polioviruses is described. In the presence of Al(+++) (1 to 100 mM) attenuated, but not virulent, strains of type 1 and type 2 polioviruses are stabilized so that they resist thermal inactivation at 50 degrees C. Differences between the attenuated and virulent type 3 strains studied were of the opposite character and less marked. By the use of optimal concentr...
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